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TRANG CHỦ Sản phẩm Hamilton Thiết bị trong phòng thí nghiệm HPLC Columns

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


 

Reversed-Phase

Reversed-Phase HPLC Columns

Hamilton reversed-phase HPLC columns combine the best characteristics of silica-based and polymeric columns to arrive at a product that is highly inert and long-lasting. Hamilton offers four polymeric and two silica-based packing materials for reversed-phase separations.

PS-DVB resins are similar in retention characteristics to silica C18s in that retention tends to increase with lipophilicity. However, subtle differences in the chemical interaction between the analyte and stationary phase can result in differential selectivity. In many cases, analytes that co-elute on a silica C18 can be resolved on a polymeric-based support. The PRP-1 consists of a 55% cross-linked PS-DVB bead containing 100 Å pores. The properties of this base material intrinsically lend themselves to reversed-phase separations with no further surface modifications. The PRP-C18 uses the PRP-1 as the base support material with the addition of octadecyl to impart characteristics more closely related to a silica-based C18, giving slightly different selectivity than the PRP-1. To make the PRP-3, the PRP-1 is modified so that the base material contains 300 Å pores, which allows for the separation of larger molecules. The PRP-h5 utilizes the PRP-1 as its base with a pentafluorinated modification, making it more hydrophobic in nature.

 

 

 

 


 

Anion Exchange

An anion exchange chromatography, the stationary bed has an ionically positive (+) charged surface while the sample ions are of negative (-) charge. This technique is used almost exclusively with ionic or ionizable samples. The stronger the negative charge on the sample, the stronger it will be attracted to the positive charge on the stationary phase, and thus the longer it will take to elute. Elution in ion chromatography is effected by mobile phase pH and ionic-strength, and, to a lesser extent, operation temperature. The ability to use the full pH range and elevated temperatures are distinct advantages compared to silica-based supports.

 

Hamilton offers six polymeric packing materials for anion exchange separations

 

 


 

Cation Exchange

In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. This technique is used almost exclusively with ionic or ionizable samples. The stronger the positive (+) charge on the sample, the stronger it will be attracted to the negative charge on the stationary phase, and thus the longer it will take to elute. The mobile phase is an aqueous buffer, where both pH and ionic strength are used to control elution time. Ion chromatography can employ harsh conditions requiring mobile phases that are at very high pH limits (> 11). Temperatures well above the normal operating conditions where silica materials fail can also be used.

 

 

 

 

 

 

 

 


 

Ion Exclusion

Ion exclusion chromatography is an alternative to ion exchange chromatography in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and nonionic compounds elute later. Mixtures of weak acids, like those in fruits and milk products, are frequently not very well separated by pure ion-exchange methods, nor in the reversed-phase mode.

PRP-X300

 

 

 

 

 

 

 

 

 


 

Custom HPLC Columns

Hamilton offers an extensive line of off-the-shelf HPLC columns with different packing material chemistries, varying particle sizes and column hardware dimensions in two hardware types—stainless steel and polyether ether ketone (PEEK) plastic. However, even with all the standard options available, customers often require a custom built column where they may need one of our stationary phases packed into a column hardware combination that is not a standard product. Custom HPLC columns offer customers the ability to have any of our stationary phases packed into most any hardware format to suit their application requirement.

 

 

 

 

 

 

 


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HPLC Application Index

 

 

 

 

 

 

 

 

 

 

 


 

USP "L" Column Listing

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


 

HPLC Resin Specifications